miRNA: 3'- -GCGgUGUACU---UCGGC---GCCGCCG-CGCa -5'
miRNA: 3'- gCGgUGUA-CuuCGGCgCCGCCG-CGca -5'
miRNA: 3'- gCGGUGUa---CuuCGGC----------GCCGCCGCgCa -5'
miRNA: 3'- gCGGU-GUACuuCGGCg-CCGCCGCgCa -5'
miRNA: 3'- gCGGUG---------UACuuCgGCGCCGCCG-CGca -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1555 | 5' | -61.4 | NC_001347.2 | + | 7821 | 0.66 | 0.781906 |
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Target: 5'- aCGUgGCGUGAcgaAGCUGguaCGGUGGCgGCGg -3' miRNA: 3'- -GCGgUGUACU---UCGGC---GCCGCCG-CGCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 182292 | 0.66 | 0.781906 |
|
Target: 5'- uGCgGCGUcGuuGCCGgGGCGGCuGCu- -3' miRNA: 3'- gCGgUGUA-CuuCGGCgCCGCCG-CGca -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 1950 | 0.66 | 0.790539 |
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Target: 5'- gGCUGCGcggcGggGCCGgcgacggggaCGGCGGCGgGg -3' miRNA: 3'- gCGGUGUa---CuuCGGC----------GCCGCCGCgCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 152990 | 0.66 | 0.790539 |
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Target: 5'- gGCCAcCAUGgcGUCGgaGGCGGUGgGc -3' miRNA: 3'- gCGGU-GUACuuCGGCg-CCGCCGCgCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 159462 | 0.66 | 0.790539 |
|
Target: 5'- gGCCGCucgaugacgAUGucGgCGCGGCGGCuGCu- -3' miRNA: 3'- gCGGUG---------UACuuCgGCGCCGCCG-CGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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