miRNA: 3'- -GCGGUGUACUUCGGCGCCGCCGCGCA- -5'
miRNA: 3'- gCGG-UGUACU-UCGGC-GCCGcCGCGCa -5'
miRNA: 3'- -GCGGUGU-ACUUCg------GCGCCGCCG-CGCa -5'
miRNA: 3'- -GCGGUGUacuUCGGCGCCG--------------CCgCGCA- -5'
miRNA: 3'- -GCGGUGuacuuCGGCGCCGC-------CGCGCa -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1555 | 5' | -61.4 | NC_001347.2 | + | 197248 | 1.07 | 0.002131 |
|
Target: 5'- aCGCCACAUGAAGCCGCGGCGGCGCGUu -3' miRNA: 3'- -GCGGUGUACUUCGGCGCCGCCGCGCA- -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 197556 | 0.69 | 0.613503 |
|
Target: 5'- gGCCgGCGUGGcGGCCGaCGGCaGCGUGc -3' miRNA: 3'- gCGG-UGUACU-UCGGC-GCCGcCGCGCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 198113 | 0.68 | 0.671201 |
|
Target: 5'- uCGUCGCGuUGAGGauagucgCGCGGUGGCaGCGg -3' miRNA: 3'- -GCGGUGU-ACUUCg------GCGCCGCCG-CGCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 200165 | 0.67 | 0.734343 |
|
Target: 5'- uCGCCACGcu-GGCCGCGGUgaggacaagaagacGGaGCGUg -3' miRNA: 3'- -GCGGUGUacuUCGGCGCCG--------------CCgCGCA- -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 228722 | 0.7 | 0.555289 |
|
Target: 5'- uCGCCACc----GCCGCGGCGgauuuccGCGCGg -3' miRNA: 3'- -GCGGUGuacuuCGGCGCCGC-------CGCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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