miRNA: 3'- gCGGugUac-----UUCGgCGCCGCCG----CGCa -5'
miRNA: 3'- -GC-GGUGUACU----UCG--GCGCCGcCGCGca -5'
miRNA: 3'- -GCGGUGUaCU--UCGGC-GCCGCc-GCGca -5'
miRNA: 3'- gCGgUGUacuUCGGCGCCGcCGCgCa -5'
miRNA: 3'- gCGG-----------UGUACUUCGGCG-CCGC----CGCGca -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1555 | 5' | -61.4 | NC_001347.2 | + | 88597 | 0.67 | 0.699763 |
|
Target: 5'- aGCCugGgcgaccuGAGCgGCGGCGGCcugcGCGg -3' miRNA: 3'- gCGGugUac-----UUCGgCGCCGCCG----CGCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 88308 | 0.67 | 0.699763 |
|
Target: 5'- aCGaCCACAUGGccgaAGUuuCGCGGCaGCGCa- -3' miRNA: 3'- -GC-GGUGUACU----UCG--GCGCCGcCGCGca -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 185540 | 0.67 | 0.690283 |
|
Target: 5'- aCGCCACGcGAuuGGCCGuCGGCGucCGCa- -3' miRNA: 3'- -GCGGUGUaCU--UCGGC-GCCGCc-GCGca -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 47136 | 0.67 | 0.690283 |
|
Target: 5'- gGCgGCAau-GGCCGCGGCaGCGgGg -3' miRNA: 3'- gCGgUGUacuUCGGCGCCGcCGCgCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 167863 | 0.68 | 0.652007 |
|
Target: 5'- uGCUaaugaugggaaACAUGAGGCCGCuGGCGucacGCGCu- -3' miRNA: 3'- gCGG-----------UGUACUUCGGCG-CCGC----CGCGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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