Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1556 | 3' | -49.4 | NC_001347.2 | + | 180758 | 0.66 | 0.999432 |
Target: 5'- --gCGCGAcUGCGUCUACGAgCUGGc -3' miRNA: 3'- aaaGCGUUuGUGCAGGUGCUaGACUu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 99998 | 0.66 | 0.999136 |
Target: 5'- --aCGCGGGCGCGgUCGCGAUgcagCUGAGc -3' miRNA: 3'- aaaGCGUUUGUGCaGGUGCUA----GACUU- -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 165549 | 0.66 | 0.998944 |
Target: 5'- --cCGCGAgaGCGCGUgCACGGUCcGGc -3' miRNA: 3'- aaaGCGUU--UGUGCAgGUGCUAGaCUu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 82207 | 0.66 | 0.998944 |
Target: 5'- cUUCGgAGACGCGuUCCGCGGUaugcgUGAc -3' miRNA: 3'- aAAGCgUUUGUGC-AGGUGCUAg----ACUu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 157926 | 0.66 | 0.998944 |
Target: 5'- gUUUUGCGAcACGUGUCCGCGAUacCUGGu -3' miRNA: 3'- -AAAGCGUU-UGUGCAGGUGCUA--GACUu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 48861 | 0.67 | 0.998449 |
Target: 5'- gUUCGCGuGCACGcgCCAaaAUCUGAc -3' miRNA: 3'- aAAGCGUuUGUGCa-GGUgcUAGACUu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 170163 | 0.68 | 0.994929 |
Target: 5'- -gUCGCGgugGACGCGUgaguaGCGGUCUGAGc -3' miRNA: 3'- aaAGCGU---UUGUGCAgg---UGCUAGACUU- -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 195790 | 0.69 | 0.989678 |
Target: 5'- ---gGCAAGUACGacaUCCGCGGUCUGAc -3' miRNA: 3'- aaagCGUUUGUGC---AGGUGCUAGACUu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 130041 | 0.7 | 0.986656 |
Target: 5'- --aCGCGAcgACGUCUGCGAUCUGc- -3' miRNA: 3'- aaaGCGUUugUGCAGGUGCUAGACuu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 138689 | 0.7 | 0.980905 |
Target: 5'- uUUUCGCGGGCggguGCGUCggcuaGCGGUCUGGu -3' miRNA: 3'- -AAAGCGUUUG----UGCAGg----UGCUAGACUu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 194646 | 0.71 | 0.976153 |
Target: 5'- --aCGCAAACACGgcgCCcgaGAUCUGGGu -3' miRNA: 3'- aaaGCGUUUGUGCa--GGug-CUAGACUU- -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 155687 | 0.72 | 0.943903 |
Target: 5'- --gCGCc-GCGCGUCUACGAUCUGu- -3' miRNA: 3'- aaaGCGuuUGUGCAGGUGCUAGACuu -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 108679 | 0.74 | 0.904939 |
Target: 5'- -gUgGCAGGCGCGUCuCACGGUCUcGGGa -3' miRNA: 3'- aaAgCGUUUGUGCAG-GUGCUAGA-CUU- -5' |
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1556 | 3' | -49.4 | NC_001347.2 | + | 196790 | 1.04 | 0.02955 |
Target: 5'- gUUUCGCAAACACGUCCACGAUCUGAAg -3' miRNA: 3'- -AAAGCGUUUGUGCAGGUGCUAGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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