Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1556 | 5' | -52.7 | NC_001347.2 | + | 142275 | 0.66 | 0.995561 |
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Target: 5'- cGCgUCGCUGAuGGACaaguuugccGCGUUgcaggagcagggCGUGGAGg -3' miRNA: 3'- -CGaAGUGGCU-CCUG---------UGCAA------------GCACCUC- -5' |
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| 1556 | 5' | -52.7 | NC_001347.2 | + | 35895 | 0.66 | 0.994862 |
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Target: 5'- -gUUC-CCGGGGAguCGUUaaaGUGGAa -3' miRNA: 3'- cgAAGuGGCUCCUguGCAAg--CACCUc -5' |
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| 1556 | 5' | -52.7 | NC_001347.2 | + | 1370 | 0.66 | 0.994076 |
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Target: 5'- --gUCGCCGcGGGACGCGUggaUCGUacuGGuGg -3' miRNA: 3'- cgaAGUGGC-UCCUGUGCA---AGCA---CCuC- -5' |
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| 1556 | 5' | -52.7 | NC_001347.2 | + | 53381 | 0.66 | 0.993196 |
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Target: 5'- gGCUUCgaaACCGAGcGACggaaACGUgcagaucagCGUGGAa -3' miRNA: 3'- -CGAAG---UGGCUC-CUG----UGCAa--------GCACCUc -5' |
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| 1556 | 5' | -52.7 | NC_001347.2 | + | 14993 | 0.7 | 0.948501 |
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Target: 5'- cGC-UCACCGAGGugGagagccguCGUUgGUGGuGg -3' miRNA: 3'- -CGaAGUGGCUCCugU--------GCAAgCACCuC- -5' |
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| 1556 | 5' | -52.7 | NC_001347.2 | + | 1690 | 0.71 | 0.929947 |
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Target: 5'- uGCUgcgCGCCGAGGAgGCGacggcgcUCGgacgGGAGc -3' miRNA: 3'- -CGAa--GUGGCUCCUgUGCa------AGCa---CCUC- -5' |
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| 1556 | 5' | -52.7 | NC_001347.2 | + | 196824 | 1.12 | 0.006777 |
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Target: 5'- cGCUUCACCGAGGACACGUUCGUGGAGa -3' miRNA: 3'- -CGAAGUGGCUCCUGUGCAAGCACCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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