miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
15569 3' -40.9 NC_004015.1 + 15 0.66 0.750577
Target:  5'- cAUUGAUCu-CGACUaCCAUuAGAAGGGc -3'
miRNA:   3'- -UAAUUAGuuGUUGAcGGUA-UUUUCCU- -5'
15569 3' -40.9 NC_004015.1 + 3339 0.66 0.705741
Target:  5'- --gGGUUAccACAGCUGCUAggacacagcaguUAAGAGGGg -3'
miRNA:   3'- uaaUUAGU--UGUUGACGGU------------AUUUUCCU- -5'
15569 3' -40.9 NC_004015.1 + 87 1.09 0.000752
Target:  5'- uAUUAAUCAACAACUGCCAUAAAAGGAu -3'
miRNA:   3'- -UAAUUAGUUGUUGACGGUAUUUUCCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.