Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1557 | 5' | -58.4 | NC_001347.2 | + | 158078 | 0.66 | 0.908832 |
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Target: 5'- uCCUCUUUGcacucagCGCCgcgCUgCGCGGcGGCc -3' miRNA: 3'- -GGAGAAACa------GCGG---GAgGCGCCaCCGc -5' |
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| 1557 | 5' | -58.4 | NC_001347.2 | + | 191032 | 0.66 | 0.902971 |
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Target: 5'- gCCUCgucGUCGUCCaucgugCCGCGGaacUGcGCGa -3' miRNA: 3'- -GGAGaaaCAGCGGGa-----GGCGCC---AC-CGC- -5' |
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| 1557 | 5' | -58.4 | NC_001347.2 | + | 38958 | 0.67 | 0.860631 |
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Target: 5'- cCCUCgggauggccggccUUGUCGCCCaCCGuCGGgucGGCu -3' miRNA: 3'- -GGAGa------------AACAGCGGGaGGC-GCCa--CCGc -5' |
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| 1557 | 5' | -58.4 | NC_001347.2 | + | 27806 | 0.67 | 0.856254 |
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Target: 5'- aCCgUCUUUGcCGCCUUCagauccucguauUGCgaaGGUGGCGg -3' miRNA: 3'- -GG-AGAAACaGCGGGAG------------GCG---CCACCGC- -5' |
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| 1557 | 5' | -58.4 | NC_001347.2 | + | 39631 | 0.68 | 0.833412 |
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Target: 5'- gCCUUc--GUCGCCCgugugCCGCGGgcgacacgcagcUGGCu -3' miRNA: 3'- -GGAGaaaCAGCGGGa----GGCGCC------------ACCGc -5' |
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| 1557 | 5' | -58.4 | NC_001347.2 | + | 71674 | 0.68 | 0.825462 |
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Target: 5'- gCCUCUuguuuucgUUGUC-CCCUUCGCGGgGGaCa -3' miRNA: 3'- -GGAGA--------AACAGcGGGAGGCGCCaCC-Gc -5' |
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| 1557 | 5' | -58.4 | NC_001347.2 | + | 143710 | 0.7 | 0.719805 |
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Target: 5'- gCUCUccGUCGCCUaugcggcaUCuCGcCGGUGGCGc -3' miRNA: 3'- gGAGAaaCAGCGGG--------AG-GC-GCCACCGC- -5' |
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| 1557 | 5' | -58.4 | NC_001347.2 | + | 196339 | 1.09 | 0.002958 |
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Target: 5'- uCCUCUUUGUCGCCCUCCGCGGUGGCGu -3' miRNA: 3'- -GGAGAAACAGCGGGAGGCGCCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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