miRNA: 3'- ugCGGUa----CCAcAUGUAAGCGG---CGUCG- -5'
miRNA: 3'- -UGCGGUACCA---------------CAUgUA----AGCGGcGUCG- -5'
miRNA: 3'- -UGCGGUAcca--CAUGuaAGCGGCGU--CG- -5'
miRNA: 3'- uGCG-GUACCACAUGUAA----GCGGCGUcg -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1559 | 3' | -54 | NC_001347.2 | + | 199323 | 0.66 | 0.976507 |
|
Target: 5'- -gGCCGgcaugGGUaUGCAUUCGUUgagGCGGCg -3' miRNA: 3'- ugCGGUa----CCAcAUGUAAGCGG---CGUCG- -5' |
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| 1559 | 3' | -54 | NC_001347.2 | + | 208722 | 0.67 | 0.963281 |
|
Target: 5'- aGCGCCGUGGUcucggucgccaggcuGUGgGUcucgUUGCC-CAGCa -3' miRNA: 3'- -UGCGGUACCA---------------CAUgUA----AGCGGcGUCG- -5' |
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| 1559 | 3' | -54 | NC_001347.2 | + | 210143 | 0.72 | 0.779276 |
|
Target: 5'- aGCGCCGUcuuucGUGCcgUCGCCGCAcuGCg -3' miRNA: 3'- -UGCGGUAcca--CAUGuaAGCGGCGU--CG- -5' |
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| 1559 | 3' | -54 | NC_001347.2 | + | 220105 | 0.67 | 0.968363 |
|
Target: 5'- uUGUaCGUGGUGUACGUUguggCGCUGCAa- -3' miRNA: 3'- uGCG-GUACCACAUGUAA----GCGGCGUcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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