miRNA: 3'- -UGCGGUACCaCAUGUAagcGGCGUCG- -5'
miRNA: 3'- -UGCGgUACCA-Ca-UGUaaGCGgCGUCG- -5'
miRNA: 3'- -UGCGGUACcacaUGUa-AgCGGCGUCG- -5'
miRNA: 3'- -UGCGGUA------CCa-----CAUGUaaGCGGCGUCG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1559 | 3' | -54 | NC_001347.2 | + | 6106 | 0.71 | 0.831779 |
|
Target: 5'- cACGCCGUGG-GUGCGUagaCUGCAGUu -3' miRNA: 3'- -UGCGGUACCaCAUGUAagcGGCGUCG- -5' |
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| 1559 | 3' | -54 | NC_001347.2 | + | 2147 | 0.71 | 0.839952 |
|
Target: 5'- cACGCgGUGGUcGggGCGgaCGCgGCAGCa -3' miRNA: 3'- -UGCGgUACCA-Ca-UGUaaGCGgCGUCG- -5' |
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| 1559 | 3' | -54 | NC_001347.2 | + | 1239 | 0.66 | 0.978844 |
|
Target: 5'- cGCGCCAUGc---GCAagUgGUCGCAGCg -3' miRNA: 3'- -UGCGGUACcacaUGUa-AgCGGCGUCG- -5' |
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| 1559 | 3' | -54 | NC_001347.2 | + | 990 | 0.69 | 0.932449 |
|
Target: 5'- aGCGCCGUcacagcGGgcacugGUGCGagUGCUGCGGCu -3' miRNA: 3'- -UGCGGUA------CCa-----CAUGUaaGCGGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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