Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1560 | 5' | -50.8 | NC_001347.2 | + | 56286 | 0.71 | 0.945481 |
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Target: 5'- gGGGAGCGGGCUugcaccgcugacgcAuuuGGAAGACUuaaGGCa -3' miRNA: 3'- -UCCUCGUCCGA--------------Uu--UCUUCUGGua-CUG- -5' |
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| 1560 | 5' | -50.8 | NC_001347.2 | + | 100300 | 0.72 | 0.900604 |
|
Target: 5'- cAGGuGCAGGCUGAGGucgcgcggcaggcAGGCCAUaGCg -3' miRNA: 3'- -UCCuCGUCCGAUUUCu------------UCUGGUAcUG- -5' |
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| 1560 | 5' | -50.8 | NC_001347.2 | + | 20857 | 0.72 | 0.915614 |
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Target: 5'- cGGGGCuGGCguuucuAGguGGCCGUGACc -3' miRNA: 3'- uCCUCGuCCGauu---UCuuCUGGUACUG- -5' |
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| 1560 | 5' | -50.8 | NC_001347.2 | + | 17233 | 0.79 | 0.608091 |
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Target: 5'- gAGGAGCAGG---GAGAGGAUgAUGACg -3' miRNA: 3'- -UCCUCGUCCgauUUCUUCUGgUACUG- -5' |
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| 1560 | 5' | -50.8 | NC_001347.2 | + | 195482 | 1.11 | 0.008164 |
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Target: 5'- gAGGAGCAGGCUAAAGAAGACCAUGACg -3' miRNA: 3'- -UCCUCGUCCGAUUUCUUCUGGUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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