Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
1560 | 5' | -50.8 | NC_001347.2 | + | 155314 | 0.66 | 0.996157 |
Target: 5'- cGGGGcCGGGCUGgucggGGGAAGAaacgugguguggggcCCGUGAg -3' miRNA: 3'- uCCUC-GUCCGAU-----UUCUUCU---------------GGUACUg -5' |
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1560 | 5' | -50.8 | NC_001347.2 | + | 47177 | 0.66 | 0.996509 |
Target: 5'- gAGGAGCgGGGCguccGAAGGCgAggaUGGCa -3' miRNA: 3'- -UCCUCG-UCCGauuuCUUCUGgU---ACUG- -5' |
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1560 | 5' | -50.8 | NC_001347.2 | + | 90660 | 0.66 | 0.996509 |
Target: 5'- gAGGAG-AGGCgGAGGAAGAuucguCCGagccUGACg -3' miRNA: 3'- -UCCUCgUCCGaUUUCUUCU-----GGU----ACUG- -5' |
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1560 | 5' | -50.8 | NC_001347.2 | + | 71240 | 0.66 | 0.997036 |
Target: 5'- ---cGCAGGUUucgucaucggaGAAGAAGACCGcGACc -3' miRNA: 3'- uccuCGUCCGA-----------UUUCUUCUGGUaCUG- -5' |
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1560 | 5' | -50.8 | NC_001347.2 | + | 198248 | 0.66 | 0.997496 |
Target: 5'- gGGGGGacuuGGCUAAAGAuuuuGAgCAgggUGACg -3' miRNA: 3'- -UCCUCgu--CCGAUUUCUu---CUgGU---ACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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