Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1560 | 5' | -50.8 | NC_001347.2 | + | 195324 | 0.67 | 0.994446 |
Target: 5'- cGGGGGCGucGUgaaGGAGGAGACUGUGAUg -3' miRNA: 3'- -UCCUCGUc-CGa--UUUCUUCUGGUACUG- -5' |
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1560 | 5' | -50.8 | NC_001347.2 | + | 191435 | 0.67 | 0.995221 |
Target: 5'- uGGAGCAGaGCagAAAGAAGAgCucGGCg -3' miRNA: 3'- uCCUCGUC-CGa-UUUCUUCUgGuaCUG- -5' |
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1560 | 5' | -50.8 | NC_001347.2 | + | 79345 | 0.68 | 0.990273 |
Target: 5'- cGGAGCacGGGCUGcuGGucuCCAUGGCc -3' miRNA: 3'- uCCUCG--UCCGAUuuCUucuGGUACUG- -5' |
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1560 | 5' | -50.8 | NC_001347.2 | + | 170366 | 0.67 | 0.992591 |
Target: 5'- cAGGAGCcGGCgc-GGGAGcuGCCGUccGACa -3' miRNA: 3'- -UCCUCGuCCGauuUCUUC--UGGUA--CUG- -5' |
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1560 | 5' | -50.8 | NC_001347.2 | + | 195482 | 1.11 | 0.008164 |
Target: 5'- gAGGAGCAGGCUAAAGAAGACCAUGACg -3' miRNA: 3'- -UCCUCGUCCGAUUUCUUCUGGUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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