miRNA: 3'- caAGGGGugGCAUGcc--CACCCCCG--GG- -5'
miRNA: 3'- caaGGGGU-GGCAUGcCCACCCCc-GGG- -5'
miRNA: 3'- -CAAGGGGUGGCAUGCCCACCCCCGGG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15603 | 5' | -62.3 | NC_004050.1 | + | 2509 | 0.68 | 0.012832 |
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Target: 5'- -aUCCCUugCGUGCcccaGUGGGGGUuuCCu -3' miRNA: 3'- caAGGGGugGCAUGcc--CACCCCCG--GG- -5' |
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| 15603 | 5' | -62.3 | NC_004050.1 | + | 1936 | 0.71 | 0.006518 |
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Target: 5'- --cCCCCAcCCGUAC-GGUGGGGaaCCCu -3' miRNA: 3'- caaGGGGU-GGCAUGcCCACCCCc-GGG- -5' |
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| 15603 | 5' | -62.3 | NC_004050.1 | + | 1960 | 1.11 | 1e-06 |
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Target: 5'- gGUUCCCCACCGUACGGGUGGGGGCCCg -3' miRNA: 3'- -CAAGGGGUGGCAUGCCCACCCCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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