Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1562 | 5' | -57.7 | NC_001347.2 | + | 142258 | 0.71 | 0.631714 |
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Target: 5'- gGCUGCGAGGCGC---UGGCCg-CGUCg -3' miRNA: 3'- -UGGCGCUCCGCGuugACUGGgaGUAG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 148824 | 0.68 | 0.835915 |
|
Target: 5'- gGCCGUGuccuuGGCGCGA--GACCCUgGUg -3' miRNA: 3'- -UGGCGCu----CCGCGUUgaCUGGGAgUAg -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 158094 | 0.72 | 0.621799 |
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Target: 5'- cGCCGCGcuGCGCGGC-GGCC-UCAUCg -3' miRNA: 3'- -UGGCGCucCGCGUUGaCUGGgAGUAG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 160805 | 0.77 | 0.344024 |
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Target: 5'- uCCGCGGGGUGCGugUGGCUCUgAUg -3' miRNA: 3'- uGGCGCUCCGCGUugACUGGGAgUAg -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 165577 | 0.67 | 0.859084 |
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Target: 5'- aACCGCGAcuccaggcGGCGCGGCa---CUUCGUCg -3' miRNA: 3'- -UGGCGCU--------CCGCGUUGacugGGAGUAG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 176429 | 0.68 | 0.827836 |
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Target: 5'- cGCCGUGuaccaccaAGGCGaca-UGGCCCUCAUg -3' miRNA: 3'- -UGGCGC--------UCCGCguugACUGGGAGUAg -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 189025 | 0.67 | 0.843821 |
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Target: 5'- gGCCGCGcgaacGCGCGug-GGCCCUCAa- -3' miRNA: 3'- -UGGCGCuc---CGCGUugaCUGGGAGUag -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 191843 | 0.68 | 0.835915 |
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Target: 5'- gACCGCGGuGGUGC--CUGucgUCCUCGUCu -3' miRNA: 3'- -UGGCGCU-CCGCGuuGACu--GGGAGUAG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 193771 | 1.09 | 0.003117 |
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Target: 5'- cACCGCGAGGCGCAACUGACCCUCAUCu -3' miRNA: 3'- -UGGCGCUCCGCGUUGACUGGGAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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