Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1562 | 5' | -57.7 | NC_001347.2 | + | 73730 | 0.67 | 0.86643 |
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Target: 5'- cGCCGCcgGAGGCGCggUU--CCCUC-UCu -3' miRNA: 3'- -UGGCG--CUCCGCGuuGAcuGGGAGuAG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 165577 | 0.67 | 0.859084 |
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Target: 5'- aACCGCGAcuccaggcGGCGCGGCa---CUUCGUCg -3' miRNA: 3'- -UGGCGCU--------CCGCGUUGacugGGAGUAG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 99542 | 0.67 | 0.843821 |
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Target: 5'- gGCCGgGucGCGCGGCagugacagcuugUGACCCgcgcUCGUCg -3' miRNA: 3'- -UGGCgCucCGCGUUG------------ACUGGG----AGUAG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 189025 | 0.67 | 0.843821 |
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Target: 5'- gGCCGCGcgaacGCGCGug-GGCCCUCAa- -3' miRNA: 3'- -UGGCGCuc---CGCGUugaCUGGGAGUag -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 925 | 0.67 | 0.843821 |
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Target: 5'- gGCCGCGGGGC-CGGagGGCCUUCGg- -3' miRNA: 3'- -UGGCGCUCCGcGUUgaCUGGGAGUag -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 148824 | 0.68 | 0.835915 |
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Target: 5'- gGCCGUGuccuuGGCGCGA--GACCCUgGUg -3' miRNA: 3'- -UGGCGCu----CCGCGUUgaCUGGGAgUAg -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 191843 | 0.68 | 0.835915 |
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Target: 5'- gACCGCGGuGGUGC--CUGucgUCCUCGUCu -3' miRNA: 3'- -UGGCGCU-CCGCGuuGACu--GGGAGUAG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 107358 | 0.68 | 0.835915 |
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Target: 5'- gGCUGCGAcgcgaaacGGUGCGAC-GACCCUUcuuuUCg -3' miRNA: 3'- -UGGCGCU--------CCGCGUUGaCUGGGAGu---AG- -5' |
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| 1562 | 5' | -57.7 | NC_001347.2 | + | 160805 | 0.77 | 0.344024 |
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Target: 5'- uCCGCGGGGUGCGugUGGCUCUgAUg -3' miRNA: 3'- uGGCGCUCCGCGUugACUGGGAgUAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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