Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15622 | 3' | -51.2 | NC_004051.1 | + | 1434 | 0.66 | 0.119479 |
Target: 5'- -gACCGuAGUaaggagUCCGGACCcaCGCCg -3' miRNA: 3'- aaUGGU-UUAgga---AGGCCUGGaaGCGG- -5' |
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15622 | 3' | -51.2 | NC_004051.1 | + | 415 | 0.66 | 0.106853 |
Target: 5'- -gACCAcuUCuuuaugaaguggCUUCCGGAaCCcgUUCGCCg -3' miRNA: 3'- aaUGGUuuAG------------GAAGGCCU-GG--AAGCGG- -5' |
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15622 | 3' | -51.2 | NC_004051.1 | + | 2279 | 0.68 | 0.082106 |
Target: 5'- gUACCaAGAUCCUUCCccacGGGCUUgaccgaUgGCCa -3' miRNA: 3'- aAUGG-UUUAGGAAGG----CCUGGA------AgCGG- -5' |
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15622 | 3' | -51.2 | NC_004051.1 | + | 2237 | 1.11 | 0.000013 |
Target: 5'- uUUACCAAAUCCUUCCGGACCUUCGCCa -3' miRNA: 3'- -AAUGGUUUAGGAAGGCCUGGAAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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