Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1563 | 5' | -59.9 | NC_001347.2 | + | 5577 | 0.66 | 0.832886 |
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Target: 5'- gGGGGCGUGccCCGCa--GAcCCCGGUu -3' miRNA: 3'- -UCCCGUACuaGGCGacgCU-GGGCCAc -5' |
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| 1563 | 5' | -59.9 | NC_001347.2 | + | 136014 | 0.67 | 0.799982 |
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Target: 5'- -cGGCcgaGGUCCGgaGCGggccgcGCCCGGUGc -3' miRNA: 3'- ucCCGua-CUAGGCgaCGC------UGGGCCAC- -5' |
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| 1563 | 5' | -59.9 | NC_001347.2 | + | 141796 | 0.68 | 0.709194 |
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Target: 5'- aAGGGCuUGAagaCCGCagGCGugCCGGc- -3' miRNA: 3'- -UCCCGuACUa--GGCGa-CGCugGGCCac -5' |
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| 1563 | 5' | -59.9 | NC_001347.2 | + | 79222 | 0.69 | 0.651367 |
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Target: 5'- uGGaGGCccugAUGAUCCGCgGCGugCUGGa- -3' miRNA: 3'- -UC-CCG----UACUAGGCGaCGCugGGCCac -5' |
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| 1563 | 5' | -59.9 | NC_001347.2 | + | 17023 | 0.69 | 0.641638 |
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Target: 5'- cAGGcgcGCGUGGUCCGCgGCGACUucgCGGa- -3' miRNA: 3'- -UCC---CGUACUAGGCGaCGCUGG---GCCac -5' |
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| 1563 | 5' | -59.9 | NC_001347.2 | + | 14022 | 0.7 | 0.631902 |
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Target: 5'- cGGGGCuGUGAaCCGCUcaGUGGCUCGGa- -3' miRNA: 3'- -UCCCG-UACUaGGCGA--CGCUGGGCCac -5' |
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| 1563 | 5' | -59.9 | NC_001347.2 | + | 193565 | 1.07 | 0.002728 |
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Target: 5'- aAGGGCAUGAUCCGCUGCGACCCGGUGc -3' miRNA: 3'- -UCCCGUACUAGGCGACGCUGGGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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