Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15638 | 5' | -53.4 | NC_004062.1 | + | 30307 | 0.66 | 0.952159 |
Target: 5'- -cAACGCaAUCGuuCCGacCGGCGAUCg -3' miRNA: 3'- ccUUGCGcUAGCu-GGCauGCCGCUAG- -5' |
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15638 | 5' | -53.4 | NC_004062.1 | + | 5757 | 0.69 | 0.870824 |
Target: 5'- uGGuAAUGCGGUCGAuCCGUuuGCGuGUGAcUCg -3' miRNA: 3'- -CC-UUGCGCUAGCU-GGCA--UGC-CGCU-AG- -5' |
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15638 | 5' | -53.4 | NC_004062.1 | + | 65557 | 0.73 | 0.697683 |
Target: 5'- cGAuCcCGAUCG-CCGUACGGuCGAUCg -3' miRNA: 3'- cCUuGcGCUAGCuGGCAUGCC-GCUAG- -5' |
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15638 | 5' | -53.4 | NC_004062.1 | + | 65708 | 1.13 | 0.002823 |
Target: 5'- gGGAACGCGAUCGACCGUACGGCGAUCg -3' miRNA: 3'- -CCUUGCGCUAGCUGGCAUGCCGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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