Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15645 | 5' | -49 | NC_004063.1 | + | 1414 | 0.66 | 0.387058 |
Target: 5'- --aCGUCGAGGGGGUGGugGg-GCUCc -3' miRNA: 3'- gugGUAGUUCUCCUAUCugUagUGGG- -5' |
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15645 | 5' | -49 | NC_004063.1 | + | 804 | 0.67 | 0.376092 |
Target: 5'- gAUCGUCAcugAGAGG-UGGugGUUGCCg -3' miRNA: 3'- gUGGUAGU---UCUCCuAUCugUAGUGGg -5' |
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15645 | 5' | -49 | NC_004063.1 | + | 1560 | 0.75 | 0.10221 |
Target: 5'- uCGCUGUCAAGAGGGgcGAUAUCGgUCg -3' miRNA: 3'- -GUGGUAGUUCUCCUauCUGUAGUgGG- -5' |
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15645 | 5' | -49 | NC_004063.1 | + | 2650 | 1.14 | 0.000084 |
Target: 5'- aCACCAUCAAGAGGAUAGACAUCACCCa -3' miRNA: 3'- -GUGGUAGUUCUCCUAUCUGUAGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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