Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1565 | 3' | -53.3 | NC_001347.2 | + | 36293 | 0.66 | 0.976851 |
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Target: 5'- uUGGGagauGCGGGguGGUgcUCGAGCGAGAGc -3' miRNA: 3'- cACUCgu--CGUCC--UCA--AGCUCGUUCUU- -5' |
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| 1565 | 3' | -53.3 | NC_001347.2 | + | 135308 | 0.67 | 0.954443 |
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Target: 5'- -gGAGCAGCGGGGGagggGAGCGucacGGGAc -3' miRNA: 3'- caCUCGUCGUCCUCaag-CUCGU----UCUU- -5' |
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| 1565 | 3' | -53.3 | NC_001347.2 | + | 142583 | 0.68 | 0.936585 |
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Target: 5'- -gGAGCAGCGGGGG--CG-GCGGGGg -3' miRNA: 3'- caCUCGUCGUCCUCaaGCuCGUUCUu -5' |
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| 1565 | 3' | -53.3 | NC_001347.2 | + | 195313 | 0.69 | 0.908736 |
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Target: 5'- cGUGAGCGGCAcGGGGgcgUCGugaAGgAGGAGa -3' miRNA: 3'- -CACUCGUCGU-CCUCa--AGC---UCgUUCUU- -5' |
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| 1565 | 3' | -53.3 | NC_001347.2 | + | 172301 | 0.71 | 0.81812 |
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Target: 5'- -gGAGCAGCaccAGGAGga-GGGCAAGGc -3' miRNA: 3'- caCUCGUCG---UCCUCaagCUCGUUCUu -5' |
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| 1565 | 3' | -53.3 | NC_001347.2 | + | 59129 | 0.71 | 0.81812 |
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Target: 5'- -aGAGCAGCGGcGGggCGAGCAccGGAc -3' miRNA: 3'- caCUCGUCGUCcUCaaGCUCGU--UCUu -5' |
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| 1565 | 3' | -53.3 | NC_001347.2 | + | 192432 | 1.06 | 0.008519 |
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Target: 5'- aGUGAGCAGCAGGAGUUCGAGCAAGAAg -3' miRNA: 3'- -CACUCGUCGUCCUCAAGCUCGUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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