miRNA: 3'- -ACcCGGGAaCACC-CGGGU------GCGGGCCu -5'
miRNA: 3'- -ACCCGGGaacaCCCGGGU---GCGGGccu -5'
miRNA: 3'- -ACCCGGGAACACCCGGGUGCgGGCCu -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15651 | 5' | -65.1 | NC_004064.1 | + | 3442 | 0.69 | 0.019811 |
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Target: 5'- aUGaGCCCUgGUGGaGCCCAaccacuUGCCCGGc -3' miRNA: 3'- -ACcCGGGAaCACC-CGGGU------GCGGGCCu -5' |
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| 15651 | 5' | -65.1 | NC_004064.1 | + | 6336 | 0.69 | 0.019177 |
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Target: 5'- gUGGGCCCacaaGGGCCCAacgUGCCCa-- -3' miRNA: 3'- -ACCCGGGaacaCCCGGGU---GCGGGccu -5' |
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| 15651 | 5' | -65.1 | NC_004064.1 | + | 6362 | 0.93 | 0.000132 |
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Target: 5'- uUGGGCCCUUGUGGGCCCACG-CCGGc -3' miRNA: 3'- -ACCCGGGAACACCCGGGUGCgGGCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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