Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15658 | 3' | -56 | NC_004065.1 | + | 111938 | 0.66 | 0.953213 |
Target: 5'- -cUCGC-CUUUCGGGAuccagaagGaCGCCCUCAa -3' miRNA: 3'- guAGCGuGAGAGUCCU--------C-GUGGGAGUg -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 198799 | 0.66 | 0.953213 |
Target: 5'- -uUCGCAaca-CAGGAGCGCgUUCGCc -3' miRNA: 3'- guAGCGUgagaGUCCUCGUGgGAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 13097 | 0.66 | 0.944927 |
Target: 5'- uCAUCGCGuCUCUCcuGAGCGCUgUUg- -3' miRNA: 3'- -GUAGCGU-GAGAGucCUCGUGGgAGug -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 130252 | 0.66 | 0.940444 |
Target: 5'- uCGUCGCuGC-CUCGGGgcGGUAUCCUcCGCa -3' miRNA: 3'- -GUAGCG-UGaGAGUCC--UCGUGGGA-GUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 229473 | 0.66 | 0.935733 |
Target: 5'- cCGUUGCGCgaacaGGGGGCGCUCgCACa -3' miRNA: 3'- -GUAGCGUGagag-UCCUCGUGGGaGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 87531 | 0.66 | 0.935733 |
Target: 5'- aUAUUGCGa--UCAGG-GCGCCCUCu- -3' miRNA: 3'- -GUAGCGUgagAGUCCuCGUGGGAGug -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 80959 | 0.67 | 0.92562 |
Target: 5'- ---gGCAC-CUCGGGGGaGCCCUcCACc -3' miRNA: 3'- guagCGUGaGAGUCCUCgUGGGA-GUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 37330 | 0.68 | 0.876034 |
Target: 5'- gAUCuGCGCUCUCGGGGGguUUCUgCAUu -3' miRNA: 3'- gUAG-CGUGAGAGUCCUCguGGGA-GUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 108394 | 0.68 | 0.861457 |
Target: 5'- --aCGCGCUCaacgccauccUCGGGGGCAUCCUg-- -3' miRNA: 3'- guaGCGUGAG----------AGUCCUCGUGGGAgug -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 19347 | 0.68 | 0.861457 |
Target: 5'- aCAUCGCGgagguCUCUCGGGAGgcgucuCGgUCUCGCg -3' miRNA: 3'- -GUAGCGU-----GAGAGUCCUC------GUgGGAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 127182 | 0.69 | 0.829953 |
Target: 5'- -cUCGCuCUUUCAGGcGgGCCCUCGg -3' miRNA: 3'- guAGCGuGAGAGUCCuCgUGGGAGUg -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 106263 | 0.7 | 0.81313 |
Target: 5'- uCGUCGUACUCgacCAGGgugcuGGCGCagaaCUCGCa -3' miRNA: 3'- -GUAGCGUGAGa--GUCC-----UCGUGg---GAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 123473 | 0.7 | 0.804477 |
Target: 5'- uGUCGCuucCUC-CAGGAa-GCCCUCACg -3' miRNA: 3'- gUAGCGu--GAGaGUCCUcgUGGGAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 47154 | 0.7 | 0.786724 |
Target: 5'- cCAUCGCGgccccCUC-CAGGccguGCAUCCUCGCc -3' miRNA: 3'- -GUAGCGU-----GAGaGUCCu---CGUGGGAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 2339 | 0.7 | 0.784918 |
Target: 5'- gCAUaCGCGCgcagCgcgugaaacgaaCAGGAGCugCCUCGCa -3' miRNA: 3'- -GUA-GCGUGa---Ga-----------GUCCUCGugGGAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 63494 | 0.7 | 0.777642 |
Target: 5'- cCGUCGCGCagC-CAGGuGCggauggccucGCCCUCGCg -3' miRNA: 3'- -GUAGCGUGa-GaGUCCuCG----------UGGGAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 127706 | 0.71 | 0.749675 |
Target: 5'- --aCGCACgugUUUCGGGGGCugCuCUCGCu -3' miRNA: 3'- guaGCGUG---AGAGUCCUCGugG-GAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 31951 | 0.71 | 0.740141 |
Target: 5'- uCAUCgGCACgCUCGGGcaccgacGCACCUUCGCu -3' miRNA: 3'- -GUAG-CGUGaGAGUCCu------CGUGGGAGUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 43102 | 0.73 | 0.65128 |
Target: 5'- -cUCGCGCUCUCcgAGGGGUcucgaccgACCCUcCGCa -3' miRNA: 3'- guAGCGUGAGAG--UCCUCG--------UGGGA-GUG- -5' |
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15658 | 3' | -56 | NC_004065.1 | + | 141572 | 0.76 | 0.447373 |
Target: 5'- ---gGCGCUCUUGGGcAGCGCCCUgACg -3' miRNA: 3'- guagCGUGAGAGUCC-UCGUGGGAgUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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