Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1566 | 5' | -57.3 | NC_001347.2 | + | 188143 | 0.66 | 0.940299 |
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Target: 5'- -gCCCAUGGuucUCUGG-CaCCGgCCGCCGa -3' miRNA: 3'- ggGGGUAUU---AGACCaGcGGC-GGUGGC- -5' |
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| 1566 | 5' | -57.3 | NC_001347.2 | + | 208723 | 0.66 | 0.930928 |
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Target: 5'- gCgCCGUGGUCUcGGUCGCCaGgCugUGg -3' miRNA: 3'- gGgGGUAUUAGA-CCAGCGG-CgGugGC- -5' |
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| 1566 | 5' | -57.3 | NC_001347.2 | + | 152517 | 0.66 | 0.935722 |
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Target: 5'- cUCCUCGUAua--GGUUGuuGCCGCUGg -3' miRNA: 3'- -GGGGGUAUuagaCCAGCggCGGUGGC- -5' |
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| 1566 | 5' | -57.3 | NC_001347.2 | + | 93971 | 0.66 | 0.939851 |
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Target: 5'- gCCCCgccgacgGUGAUUcgUGGUCGUgGCCaACUGg -3' miRNA: 3'- gGGGG-------UAUUAG--ACCAGCGgCGG-UGGC- -5' |
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| 1566 | 5' | -57.3 | NC_001347.2 | + | 26860 | 0.66 | 0.925916 |
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Target: 5'- aCgCCCGUGGUgUGGUCGgCaCGCUgggACCa -3' miRNA: 3'- -GgGGGUAUUAgACCAGC-G-GCGG---UGGc -5' |
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| 1566 | 5' | -57.3 | NC_001347.2 | + | 155955 | 0.66 | 0.930928 |
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Target: 5'- uUCUCCAUcagGAUCUGGU-GCagGCCACgCGg -3' miRNA: 3'- -GGGGGUA---UUAGACCAgCGg-CGGUG-GC- -5' |
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| 1566 | 5' | -57.3 | NC_001347.2 | + | 167954 | 0.66 | 0.94466 |
|
Target: 5'- -aCCgGgcuUCUGGUCGCCGUCGauaUCGg -3' miRNA: 3'- ggGGgUauuAGACCAGCGGCGGU---GGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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