Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15660 | 3' | -50.4 | NC_004065.1 | + | 132186 | 0.66 | 0.998573 |
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Target: 5'- cGUUCCUGUc---ACAgGUGCGgGUGAc -3' miRNA: 3'- -UAAGGAUAcgauUGUgCACGCgCACU- -5' |
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| 15660 | 3' | -50.4 | NC_004065.1 | + | 120916 | 0.66 | 0.997948 |
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Target: 5'- -gUCUcgcGC-GACACGcUGCGCGUGAa -3' miRNA: 3'- uaAGGauaCGaUUGUGC-ACGCGCACU- -5' |
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| 15660 | 3' | -50.4 | NC_004065.1 | + | 191736 | 0.67 | 0.997107 |
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Target: 5'- --aCCUggGggGACACGcGUGCGUGGu -3' miRNA: 3'- uaaGGAuaCgaUUGUGCaCGCGCACU- -5' |
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| 15660 | 3' | -50.4 | NC_004065.1 | + | 394 | 0.68 | 0.989134 |
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Target: 5'- gGUUCUaAUGCUAccACGCG-GCGCGaGAg -3' miRNA: 3'- -UAAGGaUACGAU--UGUGCaCGCGCaCU- -5' |
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| 15660 | 3' | -50.4 | NC_004065.1 | + | 77185 | 0.69 | 0.980179 |
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Target: 5'- cUUCCUGUGCc-ACAaGUGCGCGg-- -3' miRNA: 3'- uAAGGAUACGauUGUgCACGCGCacu -5' |
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| 15660 | 3' | -50.4 | NC_004065.1 | + | 689 | 1.07 | 0.01627 |
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Target: 5'- gAUUCCUAUGCUAACACGUGCGCGUGAc -3' miRNA: 3'- -UAAGGAUACGAUUGUGCACGCGCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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