Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15661 | 3' | -55 | NC_004065.1 | + | 23623 | 0.73 | 0.6709 |
Target: 5'- aGGCGGgugGAcgGGCGGCUG--GCCUCGGg -3' miRNA: 3'- -UCGCCa--CU--UCGCUGACaaCGGAGCUu -5' |
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15661 | 3' | -55 | NC_004065.1 | + | 154152 | 0.74 | 0.59873 |
Target: 5'- gGGCGGUGGcggcggcggcggcGGCGGCUGUUGCuggCUCGc- -3' miRNA: 3'- -UCGCCACU-------------UCGCUGACAACG---GAGCuu -5' |
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15661 | 3' | -55 | NC_004065.1 | + | 45457 | 0.75 | 0.568448 |
Target: 5'- uGGCGGUGGcggcagcGGCGGCgUGUUGCCccugUCGGAu -3' miRNA: 3'- -UCGCCACU-------UCGCUG-ACAACGG----AGCUU- -5' |
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15661 | 3' | -55 | NC_004065.1 | + | 869 | 1.06 | 0.006804 |
Target: 5'- gAGCGGUGAAGCGACUGUUGCCUCGAAa -3' miRNA: 3'- -UCGCCACUUCGCUGACAACGGAGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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