Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15663 | 5' | -54.6 | NC_004065.1 | + | 185337 | 0.66 | 0.968093 |
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Target: 5'- aGAUGAGCAGGAaaacguuuuagaGCUGCUUUucaaCAGGAGu -3' miRNA: 3'- -UUGCUUGUCCU------------CGACGGAGc---GUUCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 195195 | 0.72 | 0.756942 |
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Target: 5'- uGGCGAgACGGGGGCUGUCUCuGUgcGAGc -3' miRNA: 3'- -UUGCU-UGUCCUCGACGGAG-CGuuCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 201355 | 0.68 | 0.91518 |
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Target: 5'- cGCGAACugcugcGcGAGCUgcGCCUCGCcGGGGg -3' miRNA: 3'- uUGCUUGu-----C-CUCGA--CGGAGCGuUCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 211155 | 0.68 | 0.903065 |
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Target: 5'- gGAUGAACGuGAGCgaGCUggCGCAGGAGa -3' miRNA: 3'- -UUGCUUGUcCUCGa-CGGa-GCGUUCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 221118 | 0.68 | 0.920882 |
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Target: 5'- cGCGAACAGuaggcuGGCUGCCagGCA-GAGg -3' miRNA: 3'- uUGCUUGUCc-----UCGACGGagCGUuCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 222723 | 0.66 | 0.960499 |
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Target: 5'- -uCGAucguCAGGAGCU-CCUCGCccgggacccaacguAGGAGg -3' miRNA: 3'- uuGCUu---GUCCUCGAcGGAGCG--------------UUCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 228456 | 0.71 | 0.794216 |
|
Target: 5'- gGACGAACGGGAccgcGUUGCUcaUCGCGAGu- -3' miRNA: 3'- -UUGCUUGUCCU----CGACGG--AGCGUUCuc -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 229479 | 0.7 | 0.829155 |
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Target: 5'- cGCGAACAGGGG--GCgCUCGCAcaGGGGg -3' miRNA: 3'- uUGCUUGUCCUCgaCG-GAGCGU--UCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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