Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15663 | 5' | -54.6 | NC_004065.1 | + | 229479 | 0.7 | 0.829155 |
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Target: 5'- cGCGAACAGGGG--GCgCUCGCAcaGGGGg -3' miRNA: 3'- uUGCUUGUCCUCgaCG-GAGCGU--UCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 91303 | 0.71 | 0.812012 |
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Target: 5'- --gGGACAGGggaugAGCUGCCcCGCGuAGAGg -3' miRNA: 3'- uugCUUGUCC-----UCGACGGaGCGU-UCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 228456 | 0.71 | 0.794216 |
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Target: 5'- gGACGAACGGGAccgcGUUGCUcaUCGCGAGu- -3' miRNA: 3'- -UUGCUUGUCCU----CGACGG--AGCGUUCuc -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 122168 | 0.71 | 0.770218 |
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Target: 5'- uGACGAACAGGgcggugaguuuguugAGCU-CCUCGguGGGGg -3' miRNA: 3'- -UUGCUUGUCC---------------UCGAcGGAGCguUCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 195195 | 0.72 | 0.756942 |
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Target: 5'- uGGCGAgACGGGGGCUGUCUCuGUgcGAGc -3' miRNA: 3'- -UUGCU-UGUCCUCGACGGAG-CGuuCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 130894 | 0.72 | 0.747325 |
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Target: 5'- uGACGGGCGuGGGCUGCCUgGCGGcGGGc -3' miRNA: 3'- -UUGCUUGUcCUCGACGGAgCGUU-CUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 48580 | 0.74 | 0.606199 |
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Target: 5'- aAGCGGGCAcGGAGUcgGCCUC-CAGGAGg -3' miRNA: 3'- -UUGCUUGU-CCUCGa-CGGAGcGUUCUC- -5' |
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| 15663 | 5' | -54.6 | NC_004065.1 | + | 2319 | 1.07 | 0.00639 |
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Target: 5'- aAACGAACAGGAGCUGCCUCGCAAGAGg -3' miRNA: 3'- -UUGCUUGUCCUCGACGGAGCGUUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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