Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15664 | 3' | -59.5 | NC_004065.1 | + | 62324 | 0.67 | 0.807083 |
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Target: 5'- gCAGUCgaGGCUguagAGgGGCUCG-GGCUGg -3' miRNA: 3'- -GUCAGa-UCGA----UCgCCGAGCgCCGGCa -5' |
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| 15664 | 3' | -59.5 | NC_004065.1 | + | 6684 | 0.67 | 0.798539 |
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Target: 5'- aGGUacgUGGCUGGUGGaCggGUGGCCGUu -3' miRNA: 3'- gUCAg--AUCGAUCGCC-GagCGCCGGCA- -5' |
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| 15664 | 3' | -59.5 | NC_004065.1 | + | 149689 | 0.67 | 0.798539 |
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Target: 5'- gGGUCUGcaggugaacGCcgGGgGGC-CGCGGCCGc -3' miRNA: 3'- gUCAGAU---------CGa-UCgCCGaGCGCCGGCa -5' |
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| 15664 | 3' | -59.5 | NC_004065.1 | + | 29969 | 0.67 | 0.789856 |
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Target: 5'- cCGGUgCUGGCggcGGCGGCg-GCGGCaGUg -3' miRNA: 3'- -GUCA-GAUCGa--UCGCCGagCGCCGgCA- -5' |
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| 15664 | 3' | -59.5 | NC_004065.1 | + | 101127 | 0.67 | 0.772106 |
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Target: 5'- gCGGUCU-GCUuGCGGaacCGCGaGCCGUu -3' miRNA: 3'- -GUCAGAuCGAuCGCCga-GCGC-CGGCA- -5' |
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| 15664 | 3' | -59.5 | NC_004065.1 | + | 50734 | 0.67 | 0.772106 |
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Target: 5'- aGGUCUAuGCacGGCGGCUCcguGUGGUCGc -3' miRNA: 3'- gUCAGAU-CGa-UCGCCGAG---CGCCGGCa -5' |
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| 15664 | 3' | -59.5 | NC_004065.1 | + | 116136 | 0.75 | 0.359151 |
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Target: 5'- uGGUC-GGCguccugcGGCGGCUCGgGGCCGg -3' miRNA: 3'- gUCAGaUCGa------UCGCCGAGCgCCGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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