miRNA: 3'- -GUGGGCAGGUGGuCGG-------------UGcAUGGAAG- -5'
miRNA: 3'- guGGGCAGG--UGGUCGGUGCa---UGGAAG- -5'
miRNA: 3'- -GUGGGCAGGUGGUCGGUGCAUGGAAG- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15665 | 3' | -57 | NC_004065.1 | + | 23869 | 0.77 | 0.391718 |
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Target: 5'- cCGCCCGUCCACCcGCCugcccgccucaccGCcUGCCUUCc -3' miRNA: 3'- -GUGGGCAGGUGGuCGG-------------UGcAUGGAAG- -5' |
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| 15665 | 3' | -57 | NC_004065.1 | + | 162729 | 0.78 | 0.330908 |
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Target: 5'- -cCUCGUCCugGCCGGCCGCGcgagGCCUUCg -3' miRNA: 3'- guGGGCAGG--UGGUCGGUGCa---UGGAAG- -5' |
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| 15665 | 3' | -57 | NC_004065.1 | + | 6937 | 1.08 | 0.004117 |
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Target: 5'- cCACCCGUCCACCAGCCACGUACCUUCc -3' miRNA: 3'- -GUGGGCAGGUGGUCGGUGCAUGGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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