Results 101 - 107 of 107 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15666 | 3' | -52 | NC_004065.1 | + | 64914 | 0.66 | 0.995745 |
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Target: 5'- uCGCCGGGCGGGAcGCcgccucagAGACGAcgGCGc- -3' miRNA: 3'- -GUGGCUUGCUCUuCG--------UCUGCU--CGUac -5' |
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| 15666 | 3' | -52 | NC_004065.1 | + | 149908 | 0.66 | 0.995998 |
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Target: 5'- uGCCGGuuggAUGAGggGCgcgcggcucggcaggAGGCGGGCuUGa -3' miRNA: 3'- gUGGCU----UGCUCuuCG---------------UCUGCUCGuAC- -5' |
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| 15666 | 3' | -52 | NC_004065.1 | + | 19415 | 0.66 | 0.996238 |
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Target: 5'- cCGCCGGcgucggcggagguGCcGGAGGCAccgcgcugcuggcGAUGAGCGUGg -3' miRNA: 3'- -GUGGCU-------------UGcUCUUCGU-------------CUGCUCGUAC- -5' |
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| 15666 | 3' | -52 | NC_004065.1 | + | 67142 | 0.66 | 0.996354 |
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Target: 5'- gCGCCuGGACGcGAucGCGGACGAGaagGUGa -3' miRNA: 3'- -GUGG-CUUGCuCUu-CGUCUGCUCg--UAC- -5' |
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| 15666 | 3' | -52 | NC_004065.1 | + | 82380 | 0.66 | 0.996354 |
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Target: 5'- aCGCUGAAgGGGGAGCAGGgcuCGGGgAc- -3' miRNA: 3'- -GUGGCUUgCUCUUCGUCU---GCUCgUac -5' |
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| 15666 | 3' | -52 | NC_004065.1 | + | 150651 | 0.66 | 0.996354 |
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Target: 5'- gCGCgCGGACGcu--GCGGGCGuGCGUGu -3' miRNA: 3'- -GUG-GCUUGCucuuCGUCUGCuCGUAC- -5' |
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| 15666 | 3' | -52 | NC_004065.1 | + | 125381 | 0.66 | 0.996354 |
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Target: 5'- gGCUGGGCGAcGgcGCGGugGuGCcgGc -3' miRNA: 3'- gUGGCUUGCU-CuuCGUCugCuCGuaC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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