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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15666 | 5' | -47.9 | NC_004065.1 | + | 106733 | 0.66 | 0.999954 |
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Target: 5'- -cCAUGa--GAUCUgUUCUCGCGg-- -3' miRNA: 3'- uaGUACaagCUAGAgAAGAGCGCaag -5' |
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| 15666 | 5' | -47.9 | NC_004065.1 | + | 212031 | 0.66 | 0.999954 |
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Target: 5'- --gGUGUUCGAUCgac-UUCGCGUUg -3' miRNA: 3'- uagUACAAGCUAGagaaGAGCGCAAg -5' |
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| 15666 | 5' | -47.9 | NC_004065.1 | + | 223948 | 0.66 | 0.999919 |
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Target: 5'- aAUCGcgGUUCuGA-CUUUUCUCGCGUg- -3' miRNA: 3'- -UAGUa-CAAG-CUaGAGAAGAGCGCAag -5' |
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| 15666 | 5' | -47.9 | NC_004065.1 | + | 150107 | 0.66 | 0.999894 |
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Target: 5'- uUCGUGUUC-AUCUCgugccggCUCGUGgUCa -3' miRNA: 3'- uAGUACAAGcUAGAGaa-----GAGCGCaAG- -5' |
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| 15666 | 5' | -47.9 | NC_004065.1 | + | 91172 | 0.66 | 0.999894 |
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Target: 5'- cUCAUGacggucgacgCGGUCUgCUccgagCUCGCGUUCa -3' miRNA: 3'- uAGUACaa--------GCUAGA-GAa----GAGCGCAAG- -5' |
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| 15666 | 5' | -47.9 | NC_004065.1 | + | 153987 | 0.68 | 0.999432 |
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Target: 5'- cGUCGUGaUCGuugucGUCUCUg-UCGUGUUCg -3' miRNA: 3'- -UAGUACaAGC-----UAGAGAagAGCGCAAG- -5' |
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| 15666 | 5' | -47.9 | NC_004065.1 | + | 7414 | 0.87 | 0.416787 |
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Target: 5'- gAUCAUGUUCGAUCUCUUCUgC-CGUUCc -3' miRNA: 3'- -UAGUACAAGCUAGAGAAGA-GcGCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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