Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15667 | 5' | -48.9 | NC_004065.1 | + | 115660 | 0.67 | 0.999479 |
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Target: 5'- cUCGcGCCG-UAUCUACcuUUCGUGg -3' miRNA: 3'- uAGCaCGGCaAUAGAUGacAAGCACa -5' |
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| 15667 | 5' | -48.9 | NC_004065.1 | + | 215841 | 0.67 | 0.999355 |
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Target: 5'- gGUCGUGacuuCGgagUAUCUGC-GUUUGUGUa -3' miRNA: 3'- -UAGCACg---GCa--AUAGAUGaCAAGCACA- -5' |
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| 15667 | 5' | -48.9 | NC_004065.1 | + | 205824 | 0.67 | 0.999207 |
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Target: 5'- gGUUGUGCCcuGUUGUCUGC---UCGUGc -3' miRNA: 3'- -UAGCACGG--CAAUAGAUGacaAGCACa -5' |
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| 15667 | 5' | -48.9 | NC_004065.1 | + | 10294 | 0.68 | 0.998544 |
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Target: 5'- cGUCGUGCCGUUAauggucaUCUuaguagcgACUGUgaUCGUa- -3' miRNA: 3'- -UAGCACGGCAAU-------AGA--------UGACA--AGCAca -5' |
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| 15667 | 5' | -48.9 | NC_004065.1 | + | 11575 | 0.7 | 0.992685 |
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Target: 5'- uAUCGUGCCGcUAcUUGCUGUcUGUGg -3' miRNA: 3'- -UAGCACGGCaAUaGAUGACAaGCACa -5' |
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| 15667 | 5' | -48.9 | NC_004065.1 | + | 153987 | 0.7 | 0.990378 |
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Target: 5'- cGUCGUGaUCGUUGUCgucuCUG-UCGUGUu -3' miRNA: 3'- -UAGCAC-GGCAAUAGau--GACaAGCACA- -5' |
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| 15667 | 5' | -48.9 | NC_004065.1 | + | 12632 | 1.05 | 0.032939 |
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Target: 5'- uAUCGUGCCGUUAUCUACUGUUCGUGUc -3' miRNA: 3'- -UAGCACGGCAAUAGAUGACAAGCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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