Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1568 | 3' | -53 | NC_001347.2 | + | 17022 | 0.67 | 0.985478 |
Target: 5'- gCAGGcgCGcGUGGUCCGCgGCGACu--- -3' miRNA: 3'- -GUCCa-GUcUACCAGGUG-CGCUGuauc -5' |
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1568 | 3' | -53 | NC_001347.2 | + | 229130 | 0.67 | 0.983703 |
Target: 5'- aUAGGUgAGGuggguucccUGGUCCGCGCcGCAa-- -3' miRNA: 3'- -GUCCAgUCU---------ACCAGGUGCGcUGUauc -5' |
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1568 | 3' | -53 | NC_001347.2 | + | 197235 | 0.67 | 0.983703 |
Target: 5'- gAGGUCGGA-GGUC-ACGCcACAUGa -3' miRNA: 3'- gUCCAGUCUaCCAGgUGCGcUGUAUc -5' |
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1568 | 3' | -53 | NC_001347.2 | + | 155962 | 0.69 | 0.95205 |
Target: 5'- uCAGGaUCuGGUGcaggCCACGCGGCGUAu -3' miRNA: 3'- -GUCC-AGuCUACca--GGUGCGCUGUAUc -5' |
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1568 | 3' | -53 | NC_001347.2 | + | 156819 | 0.71 | 0.893682 |
Target: 5'- cCAGGacgUCGGAgGGUCCGCGCGuCGg-- -3' miRNA: 3'- -GUCC---AGUCUaCCAGGUGCGCuGUauc -5' |
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1568 | 3' | -53 | NC_001347.2 | + | 145638 | 0.76 | 0.674754 |
Target: 5'- gCGGGUguuuccCGGAgGGUCCGCGCGACAc-- -3' miRNA: 3'- -GUCCA------GUCUaCCAGGUGCGCUGUauc -5' |
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1568 | 3' | -53 | NC_001347.2 | + | 191118 | 1.08 | 0.009906 |
Target: 5'- cCAGGUCAGAUGGUCCACGCGACAUAGc -3' miRNA: 3'- -GUCCAGUCUACCAGGUGCGCUGUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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