miRNA: 3'- guCUCUAGaGCGCCGgCUG--AGCUCgC- -5'
miRNA: 3'- -GUCUCUAGaGcGCCGGCUGa---GCUCGc -5'
miRNA: 3'- guCUC-UAGAGCGC---------CGGCUGag-CUCGC- -5'
miRNA: 3'- -GUC-UCUAGAGCGcCGGCUGAGCUCgc -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15683 | 5' | -57.6 | NC_004065.1 | + | 109703 | 0.67 | 0.906589 |
|
Target: 5'- -cGAGAUCgCGCGGC-GACccUCGGGgGg -3' miRNA: 3'- guCUCUAGaGCGCCGgCUG--AGCUCgC- -5' |
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| 15683 | 5' | -57.6 | NC_004065.1 | + | 25967 | 0.67 | 0.900588 |
|
Target: 5'- cUAGGGAUCcC-CGGCCGGCggcaUGAGCc -3' miRNA: 3'- -GUCUCUAGaGcGCCGGCUGa---GCUCGc -5' |
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| 15683 | 5' | -57.6 | NC_004065.1 | + | 24782 | 0.67 | 0.894374 |
|
Target: 5'- -cGGGuAUCUCGCGccagcgcauGCCGAUgcaGAGCGa -3' miRNA: 3'- guCUC-UAGAGCGC---------CGGCUGag-CUCGC- -5' |
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| 15683 | 5' | -57.6 | NC_004065.1 | + | 118734 | 0.74 | 0.529909 |
|
Target: 5'- cCGGcAGGUCUCGCaGUCGGCUCGGGg- -3' miRNA: 3'- -GUC-UCUAGAGCGcCGGCUGAGCUCgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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