Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15684 | 3' | -59 | NC_004065.1 | + | 140387 | 0.66 | 0.893879 |
Target: 5'- gUCGGUCgCCC-GGUGUCCCC-UGUg- -3' miRNA: 3'- -GGCCGGaGGGuUCACAGGGGuGCAgu -5' |
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15684 | 3' | -59 | NC_004065.1 | + | 147204 | 0.66 | 0.880951 |
Target: 5'- uCCGGCUUCCCcgacgagcagAAGUacuUCCCCACccccggauggGUCGg -3' miRNA: 3'- -GGCCGGAGGG----------UUCAc--AGGGGUG----------CAGU- -5' |
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15684 | 3' | -59 | NC_004065.1 | + | 148319 | 0.68 | 0.80524 |
Target: 5'- cUCGGCCgccaggucgCCCAcGGUGUCgccguCCUugGUCAc -3' miRNA: 3'- -GGCCGGa--------GGGU-UCACAG-----GGGugCAGU- -5' |
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15684 | 3' | -59 | NC_004065.1 | + | 151823 | 0.67 | 0.852757 |
Target: 5'- cCCGGuCCUCuCCGAGUacugcgacGUCgCCCACaacuggGUCAu -3' miRNA: 3'- -GGCC-GGAG-GGUUCA--------CAG-GGGUG------CAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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