Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 15686 | 3' | -53.5 | NC_004065.1 | + | 187020 | 0.71 | 0.879688 |
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Target: 5'- aCCGAGUACgaGGAGaccuCGUUuggGACCAAGa -3' miRNA: 3'- -GGCUCAUGgaCCUU----GCGGa--CUGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 33528 | 0.71 | 0.879688 |
|
Target: 5'- aCCGAGUugCUGGcagauGCGCUcGGgCAGGa -3' miRNA: 3'- -GGCUCAugGACCu----UGCGGaCUgGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 126341 | 0.71 | 0.872627 |
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Target: 5'- cCCGAGUcgcaguuCCUGGAccaGCGgCUGACCu-- -3' miRNA: 3'- -GGCUCAu------GGACCU---UGCgGACUGGuuc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 166021 | 0.72 | 0.850221 |
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Target: 5'- gCGGGUGCCUGGA-CGCgUGguGCCGc- -3' miRNA: 3'- gGCUCAUGGACCUuGCGgAC--UGGUuc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 190022 | 0.73 | 0.791606 |
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Target: 5'- cCCGGGguuUCUGGGGCGCCccGCCGGGu -3' miRNA: 3'- -GGCUCau-GGACCUUGCGGacUGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 211733 | 0.73 | 0.800458 |
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Target: 5'- aCCGGGcGCCUGGcuCGCaUGGCCGAa -3' miRNA: 3'- -GGCUCaUGGACCuuGCGgACUGGUUc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 164115 | 0.75 | 0.68698 |
|
Target: 5'- aCCGAG-ACC-GGGGCGUCUG-CCGAGa -3' miRNA: 3'- -GGCUCaUGGaCCUUGCGGACuGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 29746 | 1.12 | 0.005646 |
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Target: 5'- aCCGAGUACCUGGAACGCCUGACCAAGa -3' miRNA: 3'- -GGCUCAUGGACCUUGCGGACUGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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