Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15686 | 3' | -53.5 | NC_004065.1 | + | 138328 | 0.66 | 0.987142 |
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Target: 5'- gCGAG-GCCUccucacccagggacaGGuuuCGCCUGAUCGAGa -3' miRNA: 3'- gGCUCaUGGA---------------CCuu-GCGGACUGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 69661 | 0.66 | 0.988034 |
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Target: 5'- uCCGAGUGgCUGaAGCGCgUGAagGAGa -3' miRNA: 3'- -GGCUCAUgGACcUUGCGgACUggUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 135255 | 0.66 | 0.988034 |
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Target: 5'- uCCGAGgagaUGGAGgacCGgCUGGCCAGGu -3' miRNA: 3'- -GGCUCauggACCUU---GCgGACUGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 101125 | 0.66 | 0.988034 |
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Target: 5'- -gGAGaacUACCUGGGGCgcaGCCUGgacGCCGAa -3' miRNA: 3'- ggCUC---AUGGACCUUG---CGGAC---UGGUUc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 117455 | 0.66 | 0.988034 |
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Target: 5'- cCCGccaucaccUGCCUGGcgcAACGCCUGccCCAGGg -3' miRNA: 3'- -GGCuc------AUGGACC---UUGCGGACu-GGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 124044 | 0.66 | 0.989414 |
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Target: 5'- aCGAGgACgUGG-ACGCCUGGguCCGAc -3' miRNA: 3'- gGCUCaUGgACCuUGCGGACU--GGUUc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 130800 | 0.66 | 0.990425 |
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Target: 5'- gCGAGacguccaucuuCCUGGAgcGCGCCUcGGCCGu- -3' miRNA: 3'- gGCUCau---------GGACCU--UGCGGA-CUGGUuc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 76003 | 0.66 | 0.990666 |
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Target: 5'- cCCGGGUcaGCgCUGGGGCaGCCUG-CgCAAa -3' miRNA: 3'- -GGCUCA--UG-GACCUUG-CGGACuG-GUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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