Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15686 | 3' | -53.5 | NC_004065.1 | + | 187020 | 0.71 | 0.879688 |
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Target: 5'- aCCGAGUACgaGGAGaccuCGUUuggGACCAAGa -3' miRNA: 3'- -GGCUCAUGgaCCUU----GCGGa--CUGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 190022 | 0.73 | 0.791606 |
|
Target: 5'- cCCGGGguuUCUGGGGCGCCccGCCGGGu -3' miRNA: 3'- -GGCUCau-GGACCUUGCGGacUGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 198080 | 0.68 | 0.973901 |
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Target: 5'- cCUGGG-ACCUGGAcgaccccaacgugGCGCCgcgggcgcuggUGACCAAc -3' miRNA: 3'- -GGCUCaUGGACCU-------------UGCGG-----------ACUGGUUc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 200102 | 0.67 | 0.983053 |
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Target: 5'- aCGAGUGCCUGcGGCGgUggUGGCCGu- -3' miRNA: 3'- gGCUCAUGGACcUUGCgG--ACUGGUuc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 202458 | 0.67 | 0.976647 |
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Target: 5'- gUCGGGUACCccccgGGGugGUggCUGGCCGu- -3' miRNA: 3'- -GGCUCAUGGa----CCUugCG--GACUGGUuc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 206470 | 0.67 | 0.978953 |
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Target: 5'- aCGAauGUAUC-GGAACGCCUGAuacgcUCGAGu -3' miRNA: 3'- gGCU--CAUGGaCCUUGCGGACU-----GGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 211733 | 0.73 | 0.800458 |
|
Target: 5'- aCCGGGcGCCUGGcuCGCaUGGCCGAa -3' miRNA: 3'- -GGCUCaUGGACCuuGCGgACUGGUUc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 227112 | 0.68 | 0.97416 |
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Target: 5'- aCCuuGUACCcacaGGAugGCCUG-CCGAa -3' miRNA: 3'- -GGcuCAUGGa---CCUugCGGACuGGUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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