Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15686 | 3' | -53.5 | NC_004065.1 | + | 138328 | 0.66 | 0.987142 |
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Target: 5'- gCGAG-GCCUccucacccagggacaGGuuuCGCCUGAUCGAGa -3' miRNA: 3'- gGCUCaUGGA---------------CCuu-GCGGACUGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 66125 | 0.67 | 0.985034 |
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Target: 5'- aCCGAGgcgcuggcgcgcuggGCCgcGGAccgGCGCCagcUGGCCGAGc -3' miRNA: 3'- -GGCUCa--------------UGGa-CCU---UGCGG---ACUGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 69661 | 0.66 | 0.988034 |
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Target: 5'- uCCGAGUGgCUGaAGCGCgUGAagGAGa -3' miRNA: 3'- -GGCUCAUgGACcUUGCGgACUggUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 31541 | 0.67 | 0.98248 |
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Target: 5'- gCGcuGUACCUGGAuguaccuucuagagACGCCgu-CCGAGa -3' miRNA: 3'- gGCu-CAUGGACCU--------------UGCGGacuGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 145072 | 0.67 | 0.981086 |
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Target: 5'- aCCGA-UGCCggGGAaguGCGCCgccACCGAGa -3' miRNA: 3'- -GGCUcAUGGa-CCU---UGCGGac-UGGUUC- -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 115135 | 0.67 | 0.981086 |
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Target: 5'- aCCGAGaugaugGCCUcGGAAcCGCC-GGCCGGc -3' miRNA: 3'- -GGCUCa-----UGGA-CCUU-GCGGaCUGGUUc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 25208 | 0.67 | 0.978953 |
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Target: 5'- --aAGUACCUGGuGGCGCCgauGCCGAc -3' miRNA: 3'- ggcUCAUGGACC-UUGCGGac-UGGUUc -5' |
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| 15686 | 3' | -53.5 | NC_004065.1 | + | 164115 | 0.75 | 0.68698 |
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Target: 5'- aCCGAG-ACC-GGGGCGUCUG-CCGAGa -3' miRNA: 3'- -GGCUCaUGGaCCUUGCGGACuGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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