Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15688 | 5' | -57.7 | NC_004065.1 | + | 47732 | 0.68 | 0.800212 |
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Target: 5'- -uGGCCuuGAUCACGgGGUCagagGCCAUg -3' miRNA: 3'- ccCCGG--CUAGUGCgCCAGaua-CGGUA- -5' |
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| 15688 | 5' | -57.7 | NC_004065.1 | + | 198187 | 0.69 | 0.773664 |
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Target: 5'- -cGGCCacGAUCACGUGGUCg--GCCc- -3' miRNA: 3'- ccCCGG--CUAGUGCGCCAGauaCGGua -5' |
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| 15688 | 5' | -57.7 | NC_004065.1 | + | 18060 | 0.7 | 0.736655 |
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Target: 5'- cGGGGU-GAUCACGCGGgaUCgc-GCCAa -3' miRNA: 3'- -CCCCGgCUAGUGCGCC--AGauaCGGUa -5' |
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| 15688 | 5' | -57.7 | NC_004065.1 | + | 121072 | 0.7 | 0.736655 |
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Target: 5'- aGGGCCGcgUACGCGGgcCUGUucacGCCGc -3' miRNA: 3'- cCCCGGCuaGUGCGCCa-GAUA----CGGUa -5' |
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| 15688 | 5' | -57.7 | NC_004065.1 | + | 135616 | 0.7 | 0.736655 |
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Target: 5'- -cGGCCG-UCACGCgGGUCUAcggcGCCAc -3' miRNA: 3'- ccCCGGCuAGUGCG-CCAGAUa---CGGUa -5' |
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| 15688 | 5' | -57.7 | NC_004065.1 | + | 109849 | 0.7 | 0.707978 |
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Target: 5'- gGGGGCC-AUCGCGCaGGUgcCgggcgGUGCCAc -3' miRNA: 3'- -CCCCGGcUAGUGCG-CCA--Ga----UACGGUa -5' |
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| 15688 | 5' | -57.7 | NC_004065.1 | + | 30613 | 1.07 | 0.00423 |
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Target: 5'- uGGGGCCGAUCACGCGGUCUAUGCCAUu -3' miRNA: 3'- -CCCCGGCUAGUGCGCCAGAUACGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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