Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15695 | 3' | -60.2 | NC_004065.1 | + | 40367 | 0.68 | 0.742511 |
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Target: 5'- uCCCUCCCGAcucgucgucaugUCcuCCGGGUC-CUCCGc -3' miRNA: 3'- -GGGGGGGCU------------AGacGGCCUAGaGAGGC- -5' |
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| 15695 | 3' | -60.2 | NC_004065.1 | + | 224173 | 0.69 | 0.724236 |
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Target: 5'- cUCCUCCUGggCUGCCGcuGGUCUCggCCu -3' miRNA: 3'- -GGGGGGGCuaGACGGC--CUAGAGa-GGc -5' |
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| 15695 | 3' | -60.2 | NC_004065.1 | + | 27822 | 0.69 | 0.696326 |
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Target: 5'- gCCCUCCGAUgUGCCugcGGGUgCUCggCCGc -3' miRNA: 3'- gGGGGGGCUAgACGG---CCUA-GAGa-GGC- -5' |
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| 15695 | 3' | -60.2 | NC_004065.1 | + | 113126 | 0.69 | 0.68692 |
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Target: 5'- gCCUggCCCUGAgUCUGCCGGAcgagcugCUgUCCGa -3' miRNA: 3'- -GGG--GGGGCU-AGACGGCCUa------GAgAGGC- -5' |
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| 15695 | 3' | -60.2 | NC_004065.1 | + | 82055 | 0.7 | 0.648971 |
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Target: 5'- uCCCCUCCGAguacgagaugUUGCgGGGUUUCUUCa -3' miRNA: 3'- -GGGGGGGCUa---------GACGgCCUAGAGAGGc -5' |
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| 15695 | 3' | -60.2 | NC_004065.1 | + | 57844 | 0.7 | 0.6299 |
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Target: 5'- aCCCCCCUcAggUGCCGGAUC-C-CCGa -3' miRNA: 3'- -GGGGGGGcUagACGGCCUAGaGaGGC- -5' |
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| 15695 | 3' | -60.2 | NC_004065.1 | + | 164070 | 0.72 | 0.554152 |
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Target: 5'- uCCCCCCCG--CUGCgUGGAgaacCUUUCCGa -3' miRNA: 3'- -GGGGGGGCuaGACG-GCCUa---GAGAGGC- -5' |
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| 15695 | 3' | -60.2 | NC_004065.1 | + | 56177 | 0.72 | 0.554152 |
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Target: 5'- gCUUCUCCGAcuUCUGCCGGcgCggaCUCCGu -3' miRNA: 3'- -GGGGGGGCU--AGACGGCCuaGa--GAGGC- -5' |
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| 15695 | 3' | -60.2 | NC_004065.1 | + | 39840 | 1.1 | 0.001919 |
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Target: 5'- uCCCCCCCGAUCUGCCGGAUCUCUCCGg -3' miRNA: 3'- -GGGGGGGCUAGACGGCCUAGAGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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