Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15697 | 5' | -52.7 | NC_004065.1 | + | 191610 | 0.66 | 0.99645 |
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Target: 5'- -aGACgCCUCUCccGCGAAG-UCCGugUCCc -3' miRNA: 3'- ccCUG-GGAGAGu-UGCUUCuAGGC--AGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 198737 | 0.72 | 0.911843 |
|
Target: 5'- --aACCCUCgcgugUCGuacggguCGGAGGUCCGUCCg -3' miRNA: 3'- cccUGGGAG-----AGUu------GCUUCUAGGCAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 201583 | 0.68 | 0.986407 |
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Target: 5'- uGGAucucacguCCCggCagGGCGGAGAUCCuGUCCg -3' miRNA: 3'- cCCU--------GGGa-GagUUGCUUCUAGG-CAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 202548 | 0.67 | 0.991703 |
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Target: 5'- uGGGAUCCgggaccgUGACGAucGGA-CCGUCCc -3' miRNA: 3'- -CCCUGGGaga----GUUGCU--UCUaGGCAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 211011 | 0.69 | 0.976528 |
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Target: 5'- cGGACCUUCUCuguACGA-GAUgaacgucgCCGUCa -3' miRNA: 3'- cCCUGGGAGAGu--UGCUuCUA--------GGCAGg -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 221673 | 0.7 | 0.963517 |
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Target: 5'- cGGGACCCagUCUCucuuugucaccgcCGGAucgcGAUCCGUUCg -3' miRNA: 3'- -CCCUGGG--AGAGuu-----------GCUU----CUAGGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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