Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15697 | 5' | -52.7 | NC_004065.1 | + | 34106 | 0.7 | 0.955032 |
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Target: 5'- gGGGGCUC-CUCAG-GGAGAUCgaguuCGUCCc -3' miRNA: 3'- -CCCUGGGaGAGUUgCUUCUAG-----GCAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 33578 | 0.67 | 0.987925 |
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Target: 5'- cGGGAUCCgcCUCuuCGAGGAgaauuaCGUUCg -3' miRNA: 3'- -CCCUGGGa-GAGuuGCUUCUag----GCAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 31644 | 0.66 | 0.995215 |
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Target: 5'- aGGGuCCCggcCGA-GAGGAUCCG-CCg -3' miRNA: 3'- -CCCuGGGagaGUUgCUUCUAGGCaGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 28846 | 0.66 | 0.99587 |
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Target: 5'- gGGGAUCUgUCUggaaGACGAGGGUgucCCGUCg -3' miRNA: 3'- -CCCUGGG-AGAg---UUGCUUCUA---GGCAGg -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 18152 | 0.79 | 0.549209 |
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Target: 5'- aGGcGACaguCUCUCGACGGGcGUCCGUCCg -3' miRNA: 3'- -CC-CUGg--GAGAGUUGCUUcUAGGCAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 7184 | 0.71 | 0.933304 |
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Target: 5'- uGGGCCgUCUUggUGGAGGUUUagGUCCg -3' miRNA: 3'- cCCUGGgAGAGuuGCUUCUAGG--CAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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