Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15697 | 5' | -52.7 | NC_004065.1 | + | 68513 | 0.69 | 0.974043 |
Target: 5'- aGGAUCUUCgacgcCAGCaGccGGUCCGUCCu -3' miRNA: 3'- cCCUGGGAGa----GUUG-CuuCUAGGCAGG- -5' |
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15697 | 5' | -52.7 | NC_004065.1 | + | 65910 | 0.69 | 0.976528 |
Target: 5'- cGGAUCUUCcugCGGCGGcagcggcagcGGGUCCGUCa -3' miRNA: 3'- cCCUGGGAGa--GUUGCU----------UCUAGGCAGg -5' |
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15697 | 5' | -52.7 | NC_004065.1 | + | 137275 | 0.68 | 0.978833 |
Target: 5'- aGGGCUCggucCUCAGCG-GGAUCuCGUCg -3' miRNA: 3'- cCCUGGGa---GAGUUGCuUCUAG-GCAGg -5' |
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15697 | 5' | -52.7 | NC_004065.1 | + | 113734 | 0.68 | 0.980967 |
Target: 5'- cGGugCCgacCGugGAGGAUUCGUCg -3' miRNA: 3'- cCCugGGagaGUugCUUCUAGGCAGg -5' |
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15697 | 5' | -52.7 | NC_004065.1 | + | 35894 | 0.68 | 0.982935 |
Target: 5'- gGGGACCCUgUCGcCGAuGAcaUCCccgCCg -3' miRNA: 3'- -CCCUGGGAgAGUuGCUuCU--AGGca-GG- -5' |
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15697 | 5' | -52.7 | NC_004065.1 | + | 41025 | 1.16 | 0.00442 |
Target: 5'- aGGGACCCUCUCAACGAAGAUCCGUCCg -3' miRNA: 3'- -CCCUGGGAGAGUUGCUUCUAGGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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