Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15697 | 5' | -52.7 | NC_004065.1 | + | 106215 | 0.67 | 0.989309 |
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Target: 5'- aGGACCUcuaugCUCGggaugugcaugAUGGAGGucagUCCGUCCa -3' miRNA: 3'- cCCUGGGa----GAGU-----------UGCUUCU----AGGCAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 110375 | 0.67 | 0.987925 |
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Target: 5'- uGGGA-CCUCUC-GCGcAGG-CCGUUCg -3' miRNA: 3'- -CCCUgGGAGAGuUGCuUCUaGGCAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 174677 | 0.67 | 0.987925 |
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Target: 5'- cGGGACCUcguUCUC--CGucGGGUCCGugUCCg -3' miRNA: 3'- -CCCUGGG---AGAGuuGCu-UCUAGGC--AGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 147571 | 0.67 | 0.987925 |
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Target: 5'- gGGuGACCCUCga---GAAGGUCaucauCGUCCu -3' miRNA: 3'- -CC-CUGGGAGaguugCUUCUAG-----GCAGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 35049 | 0.68 | 0.986407 |
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Target: 5'- cGGuguCCCUCUCucGACaGAAGAUCCa-CCa -3' miRNA: 3'- cCCu--GGGAGAG--UUG-CUUCUAGGcaGG- -5' |
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| 15697 | 5' | -52.7 | NC_004065.1 | + | 191289 | 0.75 | 0.765181 |
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Target: 5'- cGGGGCCCUCUCugaGGCGGAGcaggauggugCCGgCCg -3' miRNA: 3'- -CCCUGGGAGAG---UUGCUUCua--------GGCaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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