miRNA: 3'- -UCCUGGg---GCU--CGA-----CGUGCAGCUUGau -5'
miRNA: 3'- -UCCUGGggcUCGACGUGCA-GCUUgau -5'
miRNA: 3'- uCCUGGGGCUCGA----CGUgCAGCUugau -5'
miRNA: 3'- ucCUGGGGc---UCGACGUGCAgCUUGau -5'
miRNA: 3'- -UCCUGGGGCUCGac-------GUGCaGCUUGau -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15702 | 5' | -57.4 | NC_004065.1 | + | 222418 | 0.67 | 0.883784 |
|
Target: 5'- gAGGGCCgcaaCGAacGCUucgucGCGCGUCGGACg- -3' miRNA: 3'- -UCCUGGg---GCU--CGA-----CGUGCAGCUUGau -5' |
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| 15702 | 5' | -57.4 | NC_004065.1 | + | 225784 | 0.66 | 0.925747 |
|
Target: 5'- gAGGGCCagcAGCUGCGCGUgCGGAa-- -3' miRNA: 3'- -UCCUGGggcUCGACGUGCA-GCUUgau -5' |
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| 15702 | 5' | -57.4 | NC_004065.1 | + | 225834 | 0.68 | 0.806571 |
|
Target: 5'- cGGGCCUCGAGCUcgucGCGgGUCGGu--- -3' miRNA: 3'- uCCUGGGGCUCGA----CGUgCAGCUugau -5' |
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| 15702 | 5' | -57.4 | NC_004065.1 | + | 225928 | 0.73 | 0.556897 |
|
Target: 5'- -cGACCCCaaacGGCUGCGCGUgGAGCg- -3' miRNA: 3'- ucCUGGGGc---UCGACGUGCAgCUUGau -5' |
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| 15702 | 5' | -57.4 | NC_004065.1 | + | 229808 | 0.66 | 0.909089 |
|
Target: 5'- gGGGACCCUGGGCgucauuuuaUACG-CGAACg- -3' miRNA: 3'- -UCCUGGGGCUCGac-------GUGCaGCUUGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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