miRNA: 3'- uCCUGGGGCUCGA----CGUgCAGCUugau -5'
miRNA: 3'- uCCUGGGgCUCGACGugCaGC-UUGau -5'
miRNA: 3'- uCCUGGgGCUcGACGugCAGCUUGau -5'
miRNA: 3'- -UCCUGgGGCuCGA-CGUGCaGCUUGAu -5'
miRNA: 3'- -UCCUGGGGCUCGACGUGCAGCUUGAU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15702 | 5' | -57.4 | NC_004065.1 | + | 225834 | 0.68 | 0.806571 |
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Target: 5'- cGGGCCUCGAGCUcgucGCGgGUCGGu--- -3' miRNA: 3'- uCCUGGGGCUCGA----CGUgCAGCUugau -5' |
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| 15702 | 5' | -57.4 | NC_004065.1 | + | 92342 | 0.68 | 0.806571 |
|
Target: 5'- cGGGCUCgGAGCUGCugG-CGcAACg- -3' miRNA: 3'- uCCUGGGgCUCGACGugCaGC-UUGau -5' |
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| 15702 | 5' | -57.4 | NC_004065.1 | + | 23964 | 0.68 | 0.831617 |
|
Target: 5'- cGGACCgCGA-CUGCuuGUCGGGCg- -3' miRNA: 3'- uCCUGGgGCUcGACGugCAGCUUGau -5' |
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| 15702 | 5' | -57.4 | NC_004065.1 | + | 81624 | 0.67 | 0.862589 |
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Target: 5'- cAGGACgCCGuGCUcGUGCG-CGAACUc -3' miRNA: 3'- -UCCUGgGGCuCGA-CGUGCaGCUUGAu -5' |
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| 15702 | 5' | -57.4 | NC_004065.1 | + | 45345 | 1.06 | 0.004667 |
|
Target: 5'- gAGGACCCCGAGCUGCACGUCGAACUAc -3' miRNA: 3'- -UCCUGGGGCUCGACGUGCAGCUUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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