miRNA: 3'- uGUUGCUGCAgUa-AAGCgGAGGGUCa -5'
miRNA: 3'- ugUUGcUGCAGUAAAGCgGA-GGGUCa -5'
miRNA: 3'- -UGUUGCUG-CAGUAaagcGGAGGGUCa -5'
miRNA: 3'- ugUUGCUGCa--GUaAAGCGGaGGGUCA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1571 | 5' | -52 | NC_001347.2 | + | 35878 | 0.66 | 0.995271 |
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Target: 5'- cCGACGACGUgGagUUCG-UUCCCGGg -3' miRNA: 3'- uGUUGCUGCAgUa-AAGCgGAGGGUCa -5' |
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| 1571 | 5' | -52 | NC_001347.2 | + | 83357 | 0.66 | 0.995271 |
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Target: 5'- -uGACcAUGUCAUUUCGgCUgCCCAGc -3' miRNA: 3'- ugUUGcUGCAGUAAAGCgGA-GGGUCa -5' |
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| 1571 | 5' | -52 | NC_001347.2 | + | 62543 | 0.66 | 0.995271 |
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Target: 5'- gGCGGCGAUgGUCAUgu--UCUCCCAGg -3' miRNA: 3'- -UGUUGCUG-CAGUAaagcGGAGGGUCa -5' |
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| 1571 | 5' | -52 | NC_001347.2 | + | 165589 | 0.66 | 0.99652 |
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Target: 5'- -aGGCGGCGcggCAcUUCGUCgCCCAGUc -3' miRNA: 3'- ugUUGCUGCa--GUaAAGCGGaGGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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