Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1571 | 5' | -52 | NC_001347.2 | + | 38428 | 0.73 | 0.867463 |
Target: 5'- gGCGGCGACGUCGUccccgcCGCCgUCCCcGUc -3' miRNA: 3'- -UGUUGCUGCAGUAaa----GCGG-AGGGuCA- -5' |
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1571 | 5' | -52 | NC_001347.2 | + | 75850 | 0.74 | 0.807807 |
Target: 5'- gGCAACGACGUCGgccgcggggcgcagCGCCUCCuUGGUg -3' miRNA: 3'- -UGUUGCUGCAGUaaa-----------GCGGAGG-GUCA- -5' |
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1571 | 5' | -52 | NC_001347.2 | + | 20332 | 0.77 | 0.665591 |
Target: 5'- aGCAGCGGCGUCGccccagUUCGUCUCCUAa- -3' miRNA: 3'- -UGUUGCUGCAGUa-----AAGCGGAGGGUca -5' |
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1571 | 5' | -52 | NC_001347.2 | + | 189318 | 1.07 | 0.014537 |
Target: 5'- aACAACGACGUCAUUUCGCCUCCCAGUc -3' miRNA: 3'- -UGUUGCUGCAGUAAAGCGGAGGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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