Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1571 | 5' | -52 | NC_001347.2 | + | 203849 | 0.67 | 0.991234 |
Target: 5'- gGCAGCGGgGgugguaguacccaCAUUUUGCuCUCCCGGa -3' miRNA: 3'- -UGUUGCUgCa------------GUAAAGCG-GAGGGUCa -5' |
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1571 | 5' | -52 | NC_001347.2 | + | 115677 | 0.67 | 0.990518 |
Target: 5'- cAguACGGCGaUCAUgggCGCC-CCCAGc -3' miRNA: 3'- -UguUGCUGC-AGUAaa-GCGGaGGGUCa -5' |
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1571 | 5' | -52 | NC_001347.2 | + | 63785 | 0.67 | 0.990518 |
Target: 5'- uACAACGAauUCAccagcUUUCGCCUgCCCAc- -3' miRNA: 3'- -UGUUGCUgcAGU-----AAAGCGGA-GGGUca -5' |
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1571 | 5' | -52 | NC_001347.2 | + | 189318 | 1.07 | 0.014537 |
Target: 5'- aACAACGACGUCAUUUCGCCUCCCAGUc -3' miRNA: 3'- -UGUUGCUGCAGUAAAGCGGAGGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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